Combining Fourier-transform infrared spectroscopy and multivariate analysis for chemotyping of cell wall composition in Mungbean (Vigna radiata (L.) Wizcek).

Abstract

Background Dissection of complex plant cell wall structures demands a sensitive and quantitative method. FTIR is used regularly as a screening method to identify specific linkages in cell walls. However, quantification and assigning spectral bands to particular cell wall components is still a major challenge, specifically in crop species. In this study, we addressed these challenges using ATR-FTIR spectroscopy as it is a high throughput, cost-effective and non-destructive approach to understand the plant cell wall composition. This method was validated by analysing different varieties of mungbean which is one of the most important legume crops grown widely in Asia. Results Using standards and extraction of a specific component of cell wall components, we assigned 1050-1060 cm -1 and 1390-1420 cm -1 wavenumbers that can be widely used to quantify cellulose and lignin, respectively, in Arabidopsis, Populus, rice and mungbean. Also, using KBr as a diluent, we established a method that can relatively quantify the cellulose and lignin composition among different tissue types of the above species. We further used this method to quantify cellulose and lignin in field-grown mungbean genotypes. The ATR-FTIR-based study revealed the cellulose content variation ranges from 27.9% to 52.3%, and the lignin content variation ranges from 13.7% to 31.6% in mungbean genotypes. Conclusion Multivariate analysis of FT-IR data revealed differences in total cell wall (600-2000 cm -1 ), cellulose (1000-1100 cm -1 ) and lignin (1390-1420 cm -1 ) among leaf and stem of four plant species. Overall, our data suggested that ATR-FTIR can be used for the relative quantification of lignin and cellulose in different plant species. This method was successfully applied for rapid screening of cell wall composition in mungbean stem, and similarly, it can be used for screening other crops or tree species.

Plant phenotyping relevance

ATR-FTIRと多変量解析による植物細胞壁成分（セルロース・リグニン）の定量法を開発・検証し、作物遺伝子型の表現型スクリーニングに適用しているため、測定法が中心的である。

Code and data availability

The article describes ATR-FTIR phenotyping of mungbean cell wall composition, but no public phenotype datasets, spectral data, author analysis code, or trained models are deposited. The only URLs mentioned are the license, the Bruker Optics vendor site, and the generic MetaboAnalyst web tool — none are paper-specific,

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