Noninvasive In Planta Live Measurements of H 2 O 2 and Glutathione Redox Potential with Fluorescent roGFPs-Based Sensors.

Abstract

In this protocol, we present a noninvasive in planta bioimaging technique for the analysis of hydrogen peroxide (H 2 O 2 ) and glutathione redox potential in adult Arabidopsis thaliana plants. The technique is based on the use of stereo fluorescence microscopy to image A. thaliana plants expressing the two genetically encoded fluorescent sensors roGFP2-Orp1 and Grx1-roGFP2. We provide a detailed step-by-step protocol for performing low magnification imaging with mature plants grown in soil or hydroponic systems. This protocol aims to serve the scientific community by providing an accessible approach to noninvasive in planta bioimaging and data analysis.

Plant phenotyping relevance

植物体内の酸化還元状態を蛍光イメージングで非侵襲的に測定する手順を中心としたプロトコルであり、植物の生理状態を取得するフェノタイピング手法に該当する。

Code and data availability

This is a methods protocol chapter describing stereo fluorescence microscopy of roGFP2-based sensors in Arabidopsis. No public phenotype/trait datasets, plant image collections, author analysis code, or trained models are deposited or referenced. The only URLs mentioned (Fiji, Ratio Plus plugin, Micro-Manager, Napari,

No evidence-backed public reproduction asset is currently recorded.