Data-Driven Prediction of Grape Leaf Chlorophyll Content Using Hyperspectral Imaging and Convolutional Neural Networks

Abstract

Grapes, highly nutritious and flavorful fruits, require adequate chlorophyll to ensure normal growth and development. Consequently, the rapid, accurate, and efficient detection of chlorophyll content is essential. This study develops a data-driven integrated framework that combines hyperspectral imaging (HSI) and convolutional neural networks (CNNs) to predict the chlorophyll content in grape leaves, employing hyperspectral images and chlorophyll a + b content data. Initially, the VGG16-U-Net model was employed to segment the hyperspectral images of grape leaves for leaf area extraction. Subsequently, the study discussed 15 different spectral preprocessing methods, selecting fast Fourier transform (FFT) as the optimal approach. Twelve one-dimensional CNN models were subsequently developed. Experimental results revealed that the VGG16-U-Net-FFT-CNN1-1 framework developed in this study exhibited outstanding performance, achieving an R2 of 0.925 and an RMSE of 2.172, surpassing those of traditional regression models. The t-test and F-test results further confirm the statistical robustness of the VGG16-U-Net-FFT-CNN1-1 framework. This provides a basis for estimating chlorophyll content in grape leaves using HSI technology.

Plant phenotyping relevance

ブドウ葉のクロロフィル含量という植物形質を、ハイパースペクトル画像、画像分割、スペクトル前処理、CNNで推定する手法を開発・評価しており、フェノタイピング手法が中心である。

Code and data availability

The paper builds on a publicly available hyperspectral grape leaf dataset published by Ryckewaert et al. (University of Montpellier, Scientific Data), but that dataset is a cited prior work's deposit rather than an asset released by this paper. The authors' own contributions (LabelMe pixel-accurate annotation masks, VG

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