Visualizing Morphological Changes of Abscission Zone Cells in Arabidopsis by Scanning Electron Microscope.

Abstract

Scanning electron microscope (SEM) is a type of electron microscope which produces detailed images of surface structures. It has been widely used in plants and animals to study cellular structures. Here, we describe a detailed protocol to prepare samples of floral abscission zones (AZs) for SEM, as well as further image analysis. We show that it is a powerful tool to detect morphologic changes at the cellular level during the course of abscission in wild-type plants and to establish the details of phenotypic alteration in abscission mutants.

Plant phenotyping relevance

SEMによる植物の離層細胞形態の取得・画像解析プロトコルを中心に扱い、離 abscission に伴う形態変化と変異体の表現型評価へ適用しているため、植物フェノタイピング手法として含める。

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