Characterization of Root Epidermal Cell Patterning and Differentiation in Arabidopsis.

Abstract

The root epidermis of Arabidopsis thaliana has been established as a model system for elucidating the mechanisms which govern the spatial patterningAbstract and morphogenesis of plant cells. Investigations into root hairs focus on various aspects of the biology of epidermal cells, using methods specifically developed to dissect the biological question under study. Despite the large number of studies related to epidermal cell differentiation, a survey of methods to analyze the phenotypic readout resulting from environmental conditions or the genetic background of the plant has not been provided so far. This protocol describes how to analyze the spatial arrangement and morphologic characteristics of cells in the root epidermis based on whole mount roots or cross sections, using confocal, scanning electron and light microscopy. This comparison of methods aids in selecting the most suitable strategy to examine the differentiation of root epidermal cells at different developmental stages.

Plant phenotyping relevance

根表皮細胞の空間配置と形態という植物形質を、複数の顕微鏡法で解析する実験プロトコルを提示し、方法比較による選択を扱っているため、表現型取得法が中心です。

Code and data availability

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