d redefined extraction protocols utilized for crop phytochemical assessment, developed protocols, and provided technical oversight for the usage of reflectometer and offered editorial review of the manuscript. 3 Data Availability All data derived from the Bionutrient Institute methods are available publicly from our repository: https://gitlab.com/our-sci/bionutrient-institute/dataset. The data used in this manuscript covers samples submitted up to 7/31/2022 . Our Sci seeks to increase transparency and access to research via open-source hardware and software and open-access data [19]. 4 Competing Interests Statement Author G. Austic and D. Ter Avest are co-founders of OurSci, LLC, the company
Open resource ↗our-sci/bionutrient-institute/dataset · lines:108-124Unverified paper record
Validation of Low-cost Reflectometer to Identify Phytochemical Accumulation in Food Crops
11 Oct 2023 · 10.21203/rs.3.rs-3390223/v1
Abstract
Diets consisting of greater quantity/diversity of phytochemicals are correlated with reduced risk of disease. This understanding guides policy development increasing awareness of the importance of consuming fruits, grains, and vegetables. Enacted policies presume uniform concentrations of phytochemicals across crop varieties regardless of production/harvesting methods. A growing body of research suggests that concentrations of phytochemicals can fluctuate within crop varieties. Improved awareness of how cropping practices influence phytochemical concentrations are required, guiding policy development improving human health. Reliable, inexpensive laboratory equipment represents one of several barriers limiting further study of the complex interactions influencing crop phytochemical accumulation. Addressing this limitation our study validated the capacity of a low-cost Reflectometer ($500) to measure phytochemical content in selected crops, against a commercial grade laboratory spectrophotometer. Our results suggest the Reflectometer provides an accurate accounting of phytochemical content within evaluated crops. Additionally, we confirmed large variation in phytochemical content within specific crop varieties, suggesting that cultivar is but one of multiple drivers of phytochemical accumulation. Our findings indicate dramatic nutrient variations could exist across the food supply, a point whose implications are not well understood. Future studies should investigate the interactions between crop phytochemical accumulation and farm management practices that influence specific soil characteristics.
Plant phenotyping relevance
作物中の植物化学物質量を測定する低コスト反射計を商用分光光度計と比較検証しており、植物の化学的形質取得が研究の中心である。
abstractour study validated the capacity of a low-cost Reflectometer ($500) to measure phytochemical content in selected crops, against a commercial grade laboratory spectrophotometer.
abstractOur results suggest the Reflectometer provides an accurate accounting of phytochemical content within evaluated crops.
Code and data availability
The paper explicitly states that all data derived from the Bionutrient Institute methods (the crop phytochemical measurements underlying this study) are publicly available in the authors' GitLab repository, and that the automated data pipeline scripts used to calculate, merge, and QC the sample data are also publicly可用
e created to guide users through each aspect of the protocol, including Reflectometer measurements, instructions, and questions for entering metadata (ex: crop type, amount of extractant used, etc). An automated data pipeline was built using SurveyStacks API’s to merge data from each completed survey and mongoDB scripts ( https://gitlab.com/our-sci/real-food-campaign/lab-data-review-dashboard/-/tree/main ) calculated measurement outcomes. 4.2 Crop Phytochemical Variability Study 4.2.1 Crop Sample characteristics Crop samples were submitted from both producer and consumer volunteers from 2019–2022 representing 10,000 unique samples with accompanying geographical and management dat
Open resource ↗our-sci/real-food-campaign/lab-data-review-dashboard · lines:86-97cation was built using NodeJS/express with mongoDB on the Server and Vue with Vuetify on the Client. Hardware integration between SurveyStack and the Reflectometer occurs via the SurveyStack Kit mobile application written in Kotlin for Android devices. The source code for all applications is available and documented on Gitlab ( https://gitlab.com/our-sci/software/surveystack ). The Android application is available for download over the Google Play store ( https://play.google.com/store ). SurveyStack and SurveyStack Kit are licensed under the GNU General Public License v3.0. All data collection was completed using SurveyStack forms. For each data collection activity, forms were created to gui
Open resource ↗our-sci/software/surveystack · lines:86-97This is an automatically classified, unverified record. Curator approval is required before any resource enters the Catalog.