Qualitative Analysis of Lipid Peroxidation in Plants under Multiple Stress Through Schiff's Reagent: A Histochemical Approach.

Abstract

Lipid peroxidation is a physiological indicator of both biotic and abiotic stress responses, hence is often used as a biomarker to assess stress-induced cell damage or death. Here we demonstrate an easy, quick and cheap staining method to assess lipid peroxidation in plant tissues. In this methodology, Schiff's reagent, is used to assay for membrane degradation. Histochemical detection of lipid peroxidation is performed in this protocol. In brief, Schiff's reagent detects aldehydes that originate from lipid peroxides in stressful condition. Schiff's reagent is prepared and applied to plants tissue. After the reaction, plant tissue samples are rinsed with a sulfite solution to retain the staining color. From this analysis, qualitative visualization of lipid peroxidation in plant tissue is observed in the form of magenta coloration. This reagent is useful for visualization of stress induced lipid peroxidation in plants. In this protocol, Indica rice root, Assam tea root and Indian mustard seedlings are used for demonstration.

Plant phenotyping relevance

植物組織の脂質過酸化という生理状態を可視化する組織化学的測定法そのものが中心であり、単なる生物学的実験の routine measurement ではない。

Code and data availability

This is a qualitative histochemical protocol paper (Schiff's reagent staining for lipid peroxidation). No public phenotype datasets, image collections, analysis code, models, or supplementary deposits are mentioned; the paper explicitly states no data analysis is performed, and no repository or availability language or

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