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Light Microscopy, Transmission Electron Microscopy, and Immunohistochemistry Protocols for Studying Photorespiration.
Methods in molecular biology (Clifton, N.J.) · 1 Jan 2017 · 10.1007/978-1-4939-7225-8_17
Abstract
High-resolution images obtained from plant tissues processed for light microscopy, transmission electron microscopy, and immunohistochemistry have provided crucial links between plant subcellular structure and physiology during photorespiration as well as the impact of photorespiration on plant evolution and development. This chapter presents established protocols to guide researchers in the preparation of plant tissues for high-resolution imaging with a light and transmission electron microscope and detection of proteins using immunohistochemistry. Discussion of concepts and theory behind each step in the process from tissue preservation to staining of resin-embedded tissues is included to enhance the understanding of all steps in the procedure. We also include a brief protocol for quantification of cellular parameters from high-resolution images to help researchers rigorously test hypotheses.
Plant phenotyping relevance
植物組織の高解像度画像取得と、画像から細胞パラメータを定量するプロトコルを中心に扱う方法論的章であり、植物の構造・生理状態の表現型取得法に該当する。
abstractThis chapter presents established protocols to guide researchers in the preparation of plant tissues for high-resolution imaging with a light and transmission electron microscope and detection of proteins using immunohistochemistry.
abstractWe also include a brief protocol for quantification of cellular parameters from high-resolution images to help researchers rigorously test hypotheses.
Code and data availability
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