Histochemical Staining of Suberin in Plant Roots.

Abstract

Histological stains are useful tools for characterizing cell shape, arrangement and the material they are made from. Stains can be used individually or simultaneously to mark different cell structures or polymers within the same cells, and to visualize them in different colors. Histological stains can be combined with genetically-encoded fluorescent proteins, which are useful for understanding of plant development. To visualize suberin lamellae by fluorescent microscopy, we improved a histological staining procedure with the dyes Fluorol Yellow 088 and aniline blue. In the complex plant organs such as roots, suberin lamellae are deposited deep within the root on the endodermal cell wall. Our procedure yields reliable and detailed images that can be used to determine the suberin pattern in root cells. The main advantage of this protocol is its efficiency, the detailed visualization of suberin localization it generates in the root, and the possibility of returning to the confocal images to analyze and re-evaluate data if necessary.

Plant phenotyping relevance

根のスベリン局在を可視化・再解析する染色プロトコルを改良しており、植物組織の状態を取得する方法自体が中心である。

Code and data availability

This is a histochemical staining protocol (Fluorol Yellow 088/aniline blue for suberin in Arabidopsis roots) with manual ImageJ analysis. No public phenotype datasets, author image collections, analysis code, models, or supplements with such assets are mentioned. The only URLs present are bioRxiv DOIs of cited prior工作,

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