Impedance Flow Cytometry as a Tool to Analyze Microspore and Pollen Quality.

Abstract

Analyzing pollen quality in an efficient and reliable manner is of great importance to the industries involved in seed and fruit production, plant breeding, and plant research. Pollen quality parameters, viability and germination capacity, are analyzed by various staining methods or by in vitro germination assays, respectively. These methods are time-consuming, species-dependent, and require a lab environment. Furthermore, the obtained viability data are often poorly related to in vivo pollen germination and seed set. Here, we describe a quick, label-free method to analyze pollen using microfluidic chips inserted into an impedance flow cytometer (IFC). Using this approach, pollen quality parameters are determined by a single measurement in a species-independent manner. The advantage of this protocol is that pollen viability and germination can be analyzed quickly by a reliable and standardized method.

Plant phenotyping relevance

インピーダンスフローサイトメトリーを用いて、花粉の生存性・発芽能力という植物形質を迅速かつ標準化して測定する手法を開発・記述しており、表現型取得が研究の中心です。

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