Decoding the hidden half: Advances in root growth dynamics and functional architecture of maize (Zea mays L.)

Abstract

Maize (Zea mays L.) is a major cereal crop whose productivity across diverse agro-ecological environments is strongly influenced by belowground traits. The root system functions as the primary plant-soil interface, regulating water and nutrient uptake, providing mechanical support and enabling adaptive responses to abiotic and biotic stresses. Despite its central importance, maize root biology has historically received less attention than aboveground characteristics. The maize root system comprises primary, seminal, nodal and lateral roots differing in developmental origin, growth behaviour and physiological role. Key architectural traits-such as rooting depth, root growth angle and branching density-play a crucial role in root system development. These traits, along with anatomical features like cortical aerenchyma and root hair development, are governed by complex genetic networks involving regulatory genes, quantitative trait loci and hormone-mediated signalling pathways. Root growth and spatial distribution are further shaped by soil properties and agronomic practices, including irrigation and nutrient management. Recent advances in high-throughput phenotyping, three-dimensional (3D) reconstruction and artificial intelligence (AI) based image analysis technologies have enhanced quantitative assessment of root traits. This review consolidates recent progress in maize root research with emphasis on root system architecture (RSA), developmental regulation and their functional relevance to crop productivity. Uniquely, it integrates structural, genetic and phenotyping advances in maize root research into a unified framework, while explicitly linking RSA with its functional significance, an aspect often treated separately in earlier reviews. Optimising maize root systems is therefore essential for improving productivity, resource-use efficiency and agricultural sustainability under changing climatic conditions.

Plant phenotyping relevance

トウモロコシ根系研究のレビューであり、根系形態形質の定量評価に用いるハイスループット表現型解析、3D再構成、AI画像解析を明示的に扱うため、表現型解析手法レビューとして中心的です。

Code and data availability

This is a review article on maize root system architecture and phenotyping. It cites many prior studies and tools (WinRHIZO, RhizoVision Explorer, RootDetector, CT/MRI segmentation) but presents no original phenotype datasets, images, code, models, or supplements with availability statements. All referenced assets are,

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