Pre-Symptomatic Detection of Viral Infection in Tobacco Leaves Using PAM Fluorometry.

Abstract

Chlorophyll fluorescence imaging was used to study potato virus X (PVX) infection of Nicotiana benthamiana . Infection-induced changes in chlorophyll fluorescence parameters (quantum yield of photosystem II photochemistry ( Φ PSII ) and non-photochemical fluorescence quenching (NPQ)) in the non-inoculated leaf were recorded and compared with the spatial distribution of the virus detected by the fluorescence of GFP associated with the virus. We determined infection-related changes at different points of the light-induced chlorophyll fluorescence kinetics and at different days after inoculation. A slight change in the light-adapted steady-state values of Φ PSII and NPQ was observed in the infected area of the non-inoculated leaf. In contrast to the steady-state parameters, the dynamics of Φ PSII and NPQ caused by the dark-light transition in healthy and infected areas differed significantly starting from the second day after the detection of the virus in a non-inoculated leaf. The coefficients of correlation between chlorophyll fluorescence parameters and virus localization were 0.67 for Φ PSII and 0.76 for NPQ. In general, the results demonstrate the possibility of reliable pre-symptomatic detection of the spread of a viral infection using chlorophyll fluorescence imaging.

Plant phenotyping relevance

葉緑素蛍光イメージングにより、植物の感染状態を症状出現前に検出する方法を評価しており、植物状態の取得・判定が研究の中心である。

Code and data availability

植物フェノタイピング解析を再現する公開資産であることを、入力本文と直接リンクから確認できなかったため保留しました。

No evidence-backed public reproduction asset is currently recorded.