Methodology to enable high-throughput imaging of Arabidopsis seedlings on cover glass-bottom multiwell plates.

Abstract

High-throughput imaging enables rapid collection of large datasets and is used widely in many systems. However, this is not often used in plant-based systems due to issues related to the need to mount tissues and autofluorescence of plant metabolites. We therefore developed methodology enabling high-throughput imaging of Arabidopsis roots. In this system, growth media supplemented with India Ink (to block autofluorescence from cotyledons) is poured directly into multi-well coverglass-bottom plates and seedlings grown such that the roots grow down with the gravity vector and along the coverglass, effectively mounting themselves for imaging. This method enables high-throughput imaging of Arabidopsis roots.

Plant phenotyping relevance

アラビドプシス根の高スループット画像取得を可能にする培養・固定・撮像法を開発しており、植物表現型取得手法が研究の中心である。

Code and data availability

The article describes an India Ink-based high-throughput imaging methodology for Arabidopsis seedlings, but contains no public phenotype datasets, image deposits, author analysis code, or trained models. Root length measurements were made in ImageJ/FIJI (a generic tool) and no data or code availability statement with a

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