Real-Time Subcellular Imaging of Plant Signaling Molecules and Bio-Coronas by Near-Infrared Nanosensors.

Abstract

Chemical imaging at high spatiotemporal resolution is crucial for advancing plant sciences and biotechnology. We demonstrate optical nanosensors for subcellular imaging of signaling molecules (H 2 O 2 ) and lipid corona formation in plant tissues at high spatial ( 2 O 2 waves (100 μM) from plant mesophyll to stomata and pavement cells. Ca 2+ induced higher endogenous H 2 O 2 in mesophyll cells, whereas organelle electron transport chain disruptors and salt stress generated similar H 2 O 2 across all leaf cell types. Furthermore, the nanosensor quenching kinetics in photosynthetic mesophyll (0.018 s -1 ) and epidermal (0.004 s -1 ) cells enabled the detection of plant lipid corona formation. Optical nanosensors elucidate spatiotemporal dynamics of plant signaling molecules and advance our understanding of biocorona formation.

Plant phenotyping relevance

植物組織内のシグナル分子を高時空間分解能で可視化する光学ナノセンサーの開発・実証が研究の中心であり、植物の生理状態を測定する方法論に該当する。

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