Resolving subcellular sucrose concentrations in plant tissues

Abstract

Abstract Sucrose is the central unit of carbon and energy in plants. As the product of photosynthesis, it is transported from source–to–sink tissues across both short and long distances. Subcellular sucrose concentrations strongly influence rates of transport within cells, tissues, and organs. Moreover, as a central metabolite, its concentration influences the rates of many enzymatic reactions. Measuring sucrose concentration with subcellular resolution remains challenging, especially for the cytosol, which hosts many critical enzymatic reactions and, in many cells, occupies only a thin layer between the vacuole and the plasma membrane. Here, we review the methods that have been utilized to measure subcellular sucrose concentrations in plant cells. The approaches covered include microautoradiography, non-aqueous fractionation, Fourier transform infrared (FTIR) microspectroscopy, Raman microspectroscopy, mass spectrometry imaging, Förster resonance energy transfer (FRET) nanosensors, direct sampling, and theoretical modelling. We provide perspectives on the use cases for these methods and discuss developments towards resolving subcellular sugar concentrations in live tissues.

Plant phenotyping relevance

植物組織内の細胞内ショ糖濃度という生理形質を測定する手法群をレビューし、各手法の利用場面と発展を論じているため、植物フェノタイピング手法レビューに該当する。

Code and data availability

This is a review article surveying methods for measuring subcellular sucrose concentrations. All figures are reproduced from prior cited studies (Fritz et al., Moysset et al., Polit and Nazarski, Mayer et al., Kaiser et al., Guendel et al., De Moliner et al.) with publisher permission, not from this paper's own phenoty

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