Spectral technology and multispectral imaging for estimating the photosynthetic pigments and SPAD of the Chinese cabbage based on machine learning

Abstract

The contents of photosynthetic pigment, which directly affect the growth of crops, could be evaluated with spectral and multispectral imaging technologies in an accurate and rapid way. For commodity germplasm resources on the market, the estimation of photosynthetic pigments and soil and plant analyzer development (SPAD) value was accomplished using the two techniques combined with machine learning. The spectrometer used in this study employed 781 bands from 320 nm to 1100 nm, and a multispectral imaging camera was used to acquire images in visible and near-infrared. Convolutional neural network (CNN), multiple linear regression (MLR) and generalized linear model (GLM) were used to establish the machine learning models, which established by preprocessed spectral data or 4-channel multispectral images. For estimating photosynthetic pigments (chlorophyll a, chlorophyll b, total chlorophyll and carotenoids), the GLM model established by spectral data was the optimal among all the models. For the SPAD optimal estimation model, the GLM model established by the spectral data and CNN model established by the multispectral images were fair. The R² and RMSE of the CNN model validation set in estimating SPAD were 0.87 and 2.31, respectively. The R² and RMSE of the GLM model validation set in estimating SPAD were 0.88 and 2.39, respectively. By combining two techniques with different machine learning methods, a comprehensive analysis of photosynthetic pigments and SPAD was accomplished in this paper.

Plant phenotyping relevance

分光・マルチスペクトル画像と機械学習により、白菜の光合成色素およびSPADという植物形質を推定する手法を構築・検証しており、表現型取得・推定法が研究の中心である。

Code and data availability

公開状態または取得可能な本文経路を確認できませんでした。

No evidence-backed public reproduction asset is currently recorded.