Singlet Oxygen and Protochlorophyllide Detection in Arabidopsis thaliana.

Abstract

Since the recognition of the reactive oxygen species singlet oxygen ( 1 O 2 ) as a versatile signal that induces various stress responses, the mechanisms underlying 1 O 2 -induced signaling transduction pathways have become the subject of much current research. This in turn highlights the need for reliable detection methods for 1 O 2 . Here we describe a protocol for the detection of 1 O 2 using a commercially available fluorescent probe (Singlet Oxygen Sensor Green) and provide a simple method for direct visualization and quantification of the 1 O 2 -evolving photosensitizer protochlorophyllide in the Arabidopsis fluorescent mutant.

Plant phenotyping relevance

植物内の一重項酸素と光増感剤を可視化・定量する検出プロトコルが論文の中心であり、植物の生理状態を測定する手法に該当する。

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