Live imaging of Arabidopsis shoot primordia via a confocal laser scanning microscope.

Abstract

Live imaging through confocal laser scanning microscopy enables the recording, analysis, and comparison of the dynamics of shapes and gene expression patterns of plant shoot apical meristems (SAMs) or primordia. Here, we provide a protocol to describe the preparation process of imaging Arabidopsis SAMs and primordia using a confocal microscope. We describe steps for dissection, visualization of meristems using dyes and fluorescent proteins, and gain 3D morphology of meristems. We then detail analysis of shoot meristems using time-lapse imaging. For complete details on the use and execution of this protocol, please refer to Peng et al. (2022). 1 .

Plant phenotyping relevance

植物シュート頂端分裂組織の形態・動態を共焦点ライブイメージングで取得・解析する実験プロトコルであり、画像ベースの表現型取得法が中心である。

Code and data availability

This is a STAR Protocols methods paper describing live confocal imaging of Arabidopsis shoot primordia. The authors explicitly state no datasets or code were generated, and no public data, image, or code assets are provided. Figures are illustrative protocol images, not deposited datasets, and no repository URLs are in

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