CarboTag: a modular approach for live and functional imaging of plant cell walls

Abstract

Plant cells are contained inside a rigid network of cell walls. Cell walls are highly dynamic structures that act both as a structural material and as a hub for a wide range of signaling processes. Despite its crucial role in all aspects of the plant life cycle, live dynamical imaging of the cell wall and its functional properties has remained challenging. Here, we introduce CarboTag, a modular toolbox for live functional imaging of plant walls. CarboTag relies on a small molecular motif, a pyridine boronic acid, that targets its cargo to the cell wall, is non-toxic and ensures rapid tissue permeation. We designed a suite of cell wall imaging probes based on CarboTag in any desired color for multiplexing. Moreover, we created new functional reporters for live quantitative imaging of key cell wall features: network porosity, cell wall pH and the presence of reactive oxygen species. CarboTag opens the way to dynamical and quantitative mapping of cell wall responses at subcellular resolution.

Plant phenotyping relevance

植物細胞壁のライブ・定量イメージング用ツールを開発し、細胞壁の孔隙率、pH、活性酸素などの状態を取得する方法が研究の中心である。

Code and data availability

The supplied blocks describe CarboTag probe design, staining, FRAP, FLIM and imaging experiments, but contain no data availability statement, public dataset, image repository, analysis code, or trained model with an authors' public URL. No paper-specific public asset is identified.

No evidence-backed public reproduction asset is currently recorded.