Non-destructive determination of Malondialdehyde (MDA) distribution in oilseed rape leaves by laboratory scale NIR hyperspectral imaging.

Abstract

The feasibility of hyperspectral imaging with 400-1000 nm was investigated to detect malondialdehyde (MDA) content in oilseed rape leaves under herbicide stress. After comparing the performance of different preprocessing methods, linear and nonlinear calibration models, the optimal prediction performance was achieved by extreme learning machine (ELM) model with only 23 wavelengths selected by competitive adaptive reweighted sampling (CARS), and the result was R P = 0.929 and RMSEP = 2.951. Furthermore, MDA distribution map was successfully achieved by partial least squares (PLS) model with CARS. This study indicated that hyperspectral imaging technology provided a fast and nondestructive solution for MDA content detection in plant leaves.

Plant phenotyping relevance

油糧菜葉のMDA含量・分布を非破壊的に推定するハイパースペクトル画像法の検討が研究の中心であり、前処理・波長選択・校正モデル比較と分布マッピングを行っているため、植物フェノタイピング手法に該当する。

Code and data availability

The article describes hyperspectral imaging of oilseed rape and rice leaves for MDA prediction, but no public phenotype dataset, hyperspectral images, analysis code, or trained models are deposited. A Supplementary Information file is mentioned but no public URL or repository is given, and the only URL in the text is a

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