Unverified paper record
Using Genetically Encoded Fluorescent Biosensors for Quantitative In Vivo Imaging.
Methods in molecular biology (Clifton, N.J.) · 1 Jan 2021 · 10.1007/978-1-0716-0880-7_14
Abstract
Fluorescent biosensors are powerful tools for tracking analytes or cellular processes in live organisms and allowing visualization of the spatial and temporal dynamics of cellular regulators. Fluorescent protein (FP)-based biosensors are extensively employed due to their high selectivity and low invasiveness. A variety of FP-based biosensors have been engineered and applied in plant research to visualize dynamic changes in pH, redox state, concentration of molecules (ions, sugars, peptides, ATP, reactive oxygen species, and phytohormones), and activity of transporters. In this chapter, we briefly summarize reported uses of FP-based biosensors in planta and show simple methods to monitor the dynamics of intracellular Ca 2+ in Arabidopsis thaliana using a ratiometric genetically encoded Ca 2+ indicator, MatryoshCaMP6s.
Plant phenotyping relevance
植物体内の生理状態を蛍光バイオセンサーで定量可視化する方法を概説し、ArabidopsisでCa2+動態を測定する実践的方法も示すため、フェノタイピング手法が中心です。
titleUsing Genetically Encoded Fluorescent Biosensors for Quantitative In Vivo Imaging.
abstractA variety of FP-based biosensors have been engineered and applied in plant research to visualize dynamic changes in pH, redox state, concentration of molecules (ions, sugars, peptides, ATP, reactive oxygen species, and phytohormones), and activity of transporters.
abstractIn this chapter, we briefly summarize reported uses of FP-based biosensors in planta and show simple methods to monitor the dynamics of intracellular Ca 2+ in Arabidopsis thaliana using a ratiometric genetically encoded Ca 2+ indicator, MatryoshCaMP6s.
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