Light Sheet Fluorescence Microscopy Optimized for Long-Term Imaging of Arabidopsis Root Development.

Abstract

Light sheet fluorescence microscopy (LSFM) allows sustained and repeated optical sectioning of living specimens at high spatial and temporal resolution, with minimal photodamage. Here, we describe in detail both the hardware and the software elements of a live imaging method based on LSFM and optimized for tracking and 3D scanning of Arabidopsis root tips grown vertically in physiological conditions. The system is relatively inexpensive and with minimal footprint; hence it is well suited for laboratories of any size.

Plant phenotyping relevance

Arabidopsis根の長期ライブイメージングに向け、LSFMのハードウェアとソフトウェアを具体的に開発・最適化した方法論論文であり、根の3D形態・発達状態の取得が中心です。

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