Rapid diagnosis of herbicidal activity and mode of action using spectral image analysis and machine learning

Abstract

Herbicide screening requires a substantial amount of time, effort, and cost, making a new herbicide discovery expensive and time-consuming. Various diagnostic methods have been developed, but most of them are destructive and require significant time and effort to identify herbicide activity. Therefore, this study was conducted to apply spectral image analysis for early and rapid diagnosis of herbicidal activity and modes of action (MOAs). RGB, chlorophyll fluorescence (CF), and infrared (IR) thermal images were acquired after treating herbicides with different MOAs to a model plant, oilseed rape ( Brassica napus ), and analyzed using MATLAB 2021b to quantify NDI, ExG, F d /F m , and plant leaf temperature. NDI, ExG and F d /F m decreased, while plant leaf temperature increased after herbicide treatment. Distinctive spectral responses were found depending on the herbicide MOAs. PSII and PPO inhibitors showed rapid responses in IR thermal and CF images within 1 day after herbicide treatment. HPPD inhibitor showed a continuous decrease in F d /F m , while EPSPS inhibitor showed gradual changes in all spectral indices. Machine learning by Subspace Discriminant algorithm of spectral indices acquired at 6 ​h enabled the diagnosis of herbicide MOAs with 89.6 ​% accuracy, which gradually increased by adding new spectral indices acquired later time points until 3 DAT, when validation accuracy scored 100 ​%. The indices acquired at 6 ​h, and F d /F m and leaf temperature data were shown to contribute to higher accuracies of identifying herbicide MOAs. Overall test accuracy scored 87.5 ​%, verifying the possibility of diagnosing herbicide MOAs based on spectral indices. Therefore, we could conclude that herbicide activity and MOAs can be diagnosed by analyzing spectral images combined with machine learning, suggesting the possibility of high-throughput screening of herbicide MOAs using plant image analysis.

Plant phenotyping relevance

植物への除草剤処理を目的とするが、スペクトル画像から葉温度や蛍光などの植物状態を抽出し、機械学習で作用機序を診断する画像解析手法が中心であるため、植物フェノタイピング手法として含める。

Code and data availability

The paper describes spectral image analysis and machine learning for herbicide MOA diagnosis, but the supplied blocks contain no public phenotype dataset, image repository, analysis code, or trained model deposit. The only supplementary item ('Multimedia component 1') is not described as containing data or code, and no

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