A multiple ion-uptake phenotyping platform reveals shared mechanisms that affect nutrient uptake by maize roots

Abstract

Nutrient uptake is critical for crop growth and determined by root foraging in soil. Growth and branching of roots lead to effective root placement to acquire nutrients, but relatively less is known about absorption of nutrients at the root surface from the soil solution. This knowledge gap could be alleviated by understanding sources of genetic variation for short-term nutrient uptake on a root length basis. A new modular platform for high-throughput phenotyping of multiple ion uptake kinetics was designed to determine nutrient uptake rates in Zea mays . Using this system, uptake rates were characterized for the crop macronutrients nitrate, ammonium, potassium, phosphate and sulfate among the Nested Association Mapping (NAM) population founder lines. The data revealed that substantial genetic variation exists for multiple ion uptake rates in maize. Interestingly, specific nutrient uptake rates (nutrient uptake rate per length of root) were found to be both heritable and distinct from total uptake and plant size. The specific uptake rates of each nutrient were positively correlated with one another and with specific root respiration (root respiration rate per length of root), indicating that uptake is governed by shared mechanisms. We selected maize lines with high and low specific uptake rates and performed an RNA-seq analysis, which identified key regulatory components involved in nutrient uptake. The high-throughput multiple ion uptake kinetics pipeline will help further our understanding of nutrient uptake, parameterize holistic plant models, and identify breeding targets for crops with more efficient nutrient acquisition. Significance Statement Nutrient uptake is among the most limiting factors for plant growth and yet has not been used as a selection criterion in breeding. This is partly due to the lack of high-throughput phenotyping methods for measuring nutrient uptake. Here we describe a novel high-throughput phenotyping pipeline for quantification of multiple ion uptake rates. Using this new phenotyping system, our results demonstrate that specific ion uptake performance by maize plants is positively correlated among the macronutrients nitrogen, phosphorus, potassium and sulfur, and that substantial variation exists within a genetically diverse population. The findings reveal components of regulatory pathways possibly related with enhanced uptake, and confirm that nutrient uptake itself is a potential target for breeding of nutrient-efficient crops.

Plant phenotyping relevance

複数イオンの吸収速度を定量する高スループット植物表現型解析プラットフォーム自体の設計・記述が中心であり、植物の生理形質を測定する方法論研究に該当する。

Code and data availability

The paper's RhizoFlux phenotyping analysis R scripts and statistical analysis code are explicitly deposited on Zenodo with an authors' public URL (https://doi.org/10.5281/zenodo.3893945), directly reproducing the paper's ion-uptake and trait analysis. No public phenotype dataset or image deposit is stated in the blocks