A Multiplexed, Time-Resolved Assay of Root Gravitropic Bending on Agar Plates.

Abstract

The ability of roots to orient their growth relative to the vector of gravity, root gravitropism (positive gravitropism), is observed in root systems of higher plants and is an essential part of plant growth and development. While there are various methods for quantifying root gravitropism, many methods that can efficiently measure gravitropism at a reasonable throughput do not yield temporal resolution of the process, while methods that allow for high-temporal resolution are often not suitable for an efficient measurement of multiple roots. Here, we describe a method to analyze the root gravitropism activity at an increased throughput with a fine time-resolution using Arabidopsis thaliana plants.

Plant phenotyping relevance

根の重力屈性を高スループットかつ高時間分解能で定量する測定法の開発・記述が中心であり、植物形質の取得方法に該当する。

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