Potential biomonitoring of atmospheric carbon dioxide in Coffea arabica leaves using near-infrared spectroscopy and partial least squares discriminant analysis.

Abstract

The potencial of Coffea arabica leaves as bioindicators of atmospheric carbon dioxide (CO 2 ) was evaluated in a free-air carbon dioxide enrichment (FACE) experiment by using near-infrared reflectance (NIR) spectroscopy for direct analysis and partial least squares discriminant analysis (PLS-DA). A supervised classification model was built and validated from the spectra of coffee leaves grown under elevated and current CO 2 levels. PLS-DA allowed correct test set classification of 92% of the elevated-CO 2 level leaves and 100% of the current-CO 2 level leaves. The spectral bands accounting for the discrimination of the elevated-CO 2 leaves were at 1657 and 1698 nm, as indicated by the variable importance in the projection (VIP) score together with the regression coefficients. Seven months after suspension of enriched CO 2 , returning to current-CO 2 levels, new spectral measurements were made and subjected to PLS-DA analysis. The predictive model correctly classified all leaves as grown under current-CO 2 levels. The fingerprints suggest that after suspension of elevated-CO 2 , the spectral changes observed previously disappeared. The recovery could be triggered by two reasons: the relief of the stress stimulus or the perception of a return of favorable conditions. In addition, the results demonstrate that NIR spectroscopy can provide a rapid, nondestructive, and environmentally friendly method for biomonitoring leaves suffering environmental modification. Finally, C. arabica leaves associated with NIR and mathematical models have the potential to become a good biomonitoring system.

Plant phenotyping relevance

コーヒー葉の環境状態をNIR分光とPLS-DAで非破壊的に識別する手法の構築・検証が研究の中心であり、植物状態のフェノタイピング手法に該当する。

Code and data availability

The article describes NIR spectra of Coffea arabica leaves from a FACE experiment and PLS-DA modeling in MATLAB/PLS Toolbox, but contains no data availability statement, no public dataset or spectral data deposit, no author code/scripts or model checkpoints, and no supplement with such assets. Figures are only embedded

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