Surface-enhanced Raman spectroscopic chemical imaging reveals distribution of pectin and its co-localization with xyloglucan inside onion epidermal cell wall.

Abstract

The primary plant cell wall is a complex matrix composed of interconnected polysaccharides including cellulose, hemicellulose, and pectin. Changes of this dynamic polysaccharide system play a critical role during plant cell development and differentiation. A better understanding of cell wall architectures can provide insight into the plant cell development. In this study, a Raman spectroscopic imaging approach was developed to visualize the distribution of plant cell wall polysaccharides. In this approach, Surface-enhanced Raman scattering (SERS through self-assembled silver nanoparticles) was combined with Raman labels (4-Aminothiophenol. 4ATP) and targeted enzymatic hydrolysis to improve the sensitivity, specificity, and throughput of the Raman imaging technique, and to reveal the distribution of pectin and its co-localization with xyloglucan inside onion epidermal cell (OEC) wall. This technique significantly decreased the required spectral acquisition time. The resulted Raman spectra showed a high Raman signal. The resulted Raman images successfully revealed and characterized the pectin distribution and its co-localization pattern with xyloglucan in OEC wall.

Plant phenotyping relevance

植物細胞壁多糖類の分布を可視化・特徴づけるラマン分光イメージング法を開発しており、フェノタイピング手法の開発が研究の中心である。

Code and data availability

The paper's original Raman/AFM data are deposited on Mendeley (DOI: 10.17632/rc4jv3n46n.1), but no Mendeley URL is present in allowed_urls, so no paper-specific public asset can be cited with a matching URL. The allowed figure DOIs are article figures, not standalone datasets, and no author analysis code is mentioned.

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