Dual-Target Fluorescent Imaging of Hg 2+ and Cell Membrane Stress in Live Plants.

Abstract

Monitoring mercury ion (Hg 2+ ) accumulation and its phytotoxicity in plants requires analytical methods that provide both spatial and functional information beyond simple destructive quantification. We report HBTD-Hg , a dual-target fluorescent probe engineered to anchor to the cell membrane and exhibit a selective fluorescence turn-on response to Hg 2+ . This design enables simultaneous in situ imaging of Hg 2+ distribution and real-time assessment of membrane integrity in live plant tissues. The probe quantifies Hg 2+ with a detection limit of 49.7 nM. Importantly, it allows for the nondestructive tracking of Hg 2+ uptake dynamics directly through leaf imaging. Furthermore, it directly visualizes the ensuing Hg 2+ induced loss of cellular membrane integrity, including distinct vesiculation. This work provides a versatile tool for the real-time assessment of heavy metal stress, effectively linking environmental ion detection to observable cytological damage in plants.

Plant phenotyping relevance

植物組織の膜完全性・細胞障害を生体蛍光イメージングで可視化・追跡するプローブを開発しており、植物ストレス状態の取得法が中心的である。

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