Determination of lead content in oilseed rape leaves in silicon-free and silicon environments based on deep transfer learning and fluorescence hyperspectral imaging.

Abstract

The ability of fluorescence hyperspectral imaging to predict heavy metal lead (Pb) concentration in oilseed rape leaves was studied in silicon-free and silicon environments. Further, the transfer stacked convolution auto-encoder (T-SCAE) algorithm was proposed based on the stacked convolution auto-encoder (SCAE) algorithm. Fluorescence hyperspectral images of oilseed rape leaves under different Pb stress contents were obtained in the silicon-free and silicon environments. The entire region of oilseed rape leaves was chosen as the region of interest (ROI) to obtain fluorescence spectra. First of all, standard normalized variable (SNV) algorithm was implemented as the preferred preprocessing method, and the fluorescence spectral data processed by SNV was utilized for further analysis. Further, SCAE was used to reduce the dimensionality of the best pre-processed spectral data, and compared with the traditional dimensionality reduction algorithm. Finally, the optimal SCAE deep learning network was transferred to obtain the T-SCAE model to verify the transferability between the deep learning models in silicon-free and silicon environments. The results show that the SVR model based on the depth features extracted by SCAE has the best performance in predicting different Pb concentrations in silicon-free or silicon environments, and the coefficient of determination (R p 2 ), root mean square error (RMSEP) and residual predictive deviation (RPD) of prediction set in silicon-free or silicon environments were 0.9374, 0.02071 mg/kg and 3.268, and 0.9416, 0.01898 mg/kg and 3.316, respectively. Moreover, the SVR model based on the depth feature extracted by T-SCAE has the best performance in predicting different Pb concentrations in silicon-free and silicon environments, and the R p 2 , RMSEP and RPD of the optimal prediction set were 0.9385, 0.02017 mg/kg and 3.291, respectively. The combination of hyperspectral fluorescence imaging and deep transfer learning algorithm can effectively detect different Pb concentrations in oilseed rape leaves in both non-silicon environment and silicon environment.

Plant phenotyping relevance

蛍光ハイパースペクトル画像と深層転移学習を用いて、ナタネ葉のPb濃度という植物状態を推定する手法を開発・検証しており、表現型取得・抽出が研究の中心である。

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