Deep machine learning for cell segmentation and quantitative analysis of radial plant growth

Abstract

Plants produce the major part of terrestrial biomass and are long-term deposits of atmospheric carbon. This capacity is to a large extent due to radial growth of woody species - a process driven by cambium stem cells located in distinct niches of shoot and root axes. In the model species Arabidopsis thaliana, thousands of cells are produced by the cambium in radial orientation generating a complex organ anatomy enabling long-distance transport, mechanical support and protection against biotic and abiotic stressors. These complex organ dynamics make a comprehensive and unbiased analysis of radial growth challenging and asks for tools for automated quantification. Here, we combined the recently developed PlantSeg and MorphographX image analysis tools, to characterize tissue morphogenesis of the Arabidopsis hypocotyl. After sequential training of segmentation models on ovules, shoot apical meristems and adult hypocotyls using deep machine learning, followed by the training of cell type classification models, our pipeline segments complex images of transverse hypocotyl sections with high accuracy and classifies central hypocotyl cell types. By applying our pipeline on both wild type and phloem intercalated with xylem (pxy) mutants, we also show that this strategy faithfully detects major anatomical aberrations. Collectively, we conclude that our established pipeline is a powerful phenotyping tool comprehensively extracting cellular parameters and providing access to tissue topology during radial plant growth.

Plant phenotyping relevance

深層学習による細胞セグメンテーション・分類パイプラインを開発し、植物組織の形態形成と細胞パラメータを自動抽出する研究であり、植物表現型取得手法が中心である。

Code and data availability

公開論文であることは確認できましたが、現在の公式API・許可済み取得経路では本文を自動取得できませんでした。

No evidence-backed public reproduction asset is currently recorded.