Portable Raman leaf-clip sensor for rapid detection of plant stress

Abstract

Abstract Precision agriculture requires new technologies for rapid diagnosis of plant stresses, such as nutrient deficiency and drought, before the onset of visible symptoms and subsequent yield loss. Here, we demonstrate a portable Raman probe that clips around a leaf for rapid, in vivo spectral analysis of plant metabolites including carotenoids and nitrates. We use the leaf-clip Raman sensor for early diagnosis of nitrogen deficiency of the model plant Arabidopsis thaliana as well as two important vegetable crops, Pak Choi ( Brassica rapa chinensis ) and Choy Sum ( Brassica rapa var. parachinensis ) . In vivo measurements using the portable leaf-clip Raman sensor under full-light growth conditions were consistent with those obtained with a benchtop Raman spectrometer measurements on leaf-sections under laboratory conditions. The portable leaf-clip Raman sensor offers farmers and plant scientists a new precision agriculture tool for early diagnosis and real-time monitoring of plant stresses in field conditions.

Plant phenotyping relevance

葉クリップ型Ramanセンサーを開発し、植物の栄養欠乏・乾燥ストレスを早期診断する手法として検証しており、植物ストレス状態の取得が中心である。

Code and data availability

The article describes a portable Raman leaf-clip sensor and MATLAB spectral processing, but the supplied blocks contain no public dataset, image, code, or model deposit. No data availability statement or author-provided public URL appears; only supplementary figure/table files (DOCX) are mentioned, which are not stated

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