In Vivo Imaging of Microtubule Organization in Dividing Giant Cell.

Abstract

Mitosis which is a major step during plant development can also be observed in physiopathological conditions. During the compatible interaction between the root-knot nematode Meloidogyne incognita and its host Arabidopsis, the pathogen induce through repeated divisions without complete cytokinesis the formation of hypertrophied and multinucleate feeding cells, named giant cells. Due to the presence of hypertrophied plant cell material surrounding the giant cells, classical live cell imaging gave therefore very poor resolution. Here, we describe a protocol which allows the in vivo observation of the mitotic apparatus in developing giant cells using confocal imaging of vibrosliced tissues. This approach can also be used to visualize in vivo other cellular processes occurring in different steps of giant cells.

Plant phenotyping relevance

植物の発達過程における細胞構造を可視化する共焦点イメージング手法のプロトコル開発が中心であり、単なる生物学的測定ではない。

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