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VE-MLM: A variable endmember-based multilinear mixing framework for crop FAPAR estimation using UAV multispectral imagery

Plant Phenomics · 1 Jun 2026 · 10.1016/j.plaphe.2026.100202

Abstract

The fraction of absorbed photosynthetically active radiation (FAPAR) is critical for characterizing crop photosynthetic capacity and growth status. Remote sensing technology based on unmanned aerial vehicles (UAVs) enables efficient estimation of FAPAR, but multiple scattering and transmission in the complex and dynamically changing crop canopy and background limit the accuracy of vegetation index (VI)-based methods. This study proposed an adaptive spectral unmixing framework VE-MLM for the multi-layer mixed scenarios, comprising three modules: (1) Variable Endmember Extraction , building a spectral library of foreground (crop) and background endmembers, by extracting pure pixels on the R-NIR feature space and reducing redundancy using k-means and iterative endmember selection algorithm; (2) Iterative Unmixing , iterating over foreground-background endmember combinations as input of the multilinear mixing model (MLM) pixel by pixel; (3) Optimal Selection , selecting the optimal combination according to RMSE and outputting corresponding canopy abundance A f . Taking sorghum and rice as study objects, this study collected UAV multispectral images and field-measured FAPAR at multiple periods to validate the advantages of VE-MLM. The results demonstrated that compared to fixed-endmembers and linear/bilinear mixing models, VE-MLM always achieved excellent unmixing performance, effectively quantifying canopy contributions. The derived A f mitigated the saturation and background interference that commonly existed in VI-based regression models and exhibited a higher correlation with FAPAR (sorghum: R 2 = 0.900, rRMSE = 7.753%; rice: R 2 = 0.807, rRMSE = 2.200%). In conclusion, VE-MLM has a great potential to address spectral variability, dynamic changes, and scene complexity in crop growth scenarios, providing a more accurate and generalizable approach for sorghum and rice FAPAR estimation in precision agriculture.

Plant phenotyping relevance

UAVマルチスペクトル画像から作物キャノピーのFAPARを推定するスペクトルアンミキシング手法を開発し、ソルガムとイネで実測値により検証しており、植物表現型取得が中心である。

abstractThis study proposed an adaptive spectral unmixing framework VE-MLM for the multi-layer mixed scenarios
abstractthis study collected UAV multispectral images and field-measured FAPAR at multiple periods to validate the advantages of VE-MLM
abstractproviding a more accurate and generalizable approach for sorghum and rice FAPAR estimation

Code and data availability

The paper's UAV multispectral imagery, field-measured FAPAR data, and VE-MLM analysis are not publicly deposited. The only availability statement says data can be obtained from the corresponding author upon reasonable request. No public code, model, or dataset URLs are provided; the DOI link is the article itself, nota

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