Nondestructive presymptomatic detection of blue-fluorescing substances in tomato leaves infected with Ralstonia solanacearum using a polyvinyl alcohol hydrogel.

Abstract

This study proposes a nondestructive technique for the presymptomatic detection of pathogenic infections of plants, aiming to effectively prevent and control plant diseases in agriculture. The present and previous studies indicated that an increase in blue-fluorescing substances, including chlorogenic acid, in tomato leaves is a promising biomarker of infection with the pathogenic soil bacterium Ralstonia solanacearum. A soft and adhesive polyvinyl alcohol (PVA) hydrogel conformably adhered to the hydrophobic surface of the tomato leaf with a complex topography, mediated by non-volatile glycerol, enabling effective extraction of blue-fluorescing substances in a nondestructive manner. The fluorescence intensity of the PVA hydrogel increased a few days before the appearance of visible symptoms of bacterial wilt. This technique is expected to become a fundamental technology for the early detection of plant diseases.

Plant phenotyping relevance

トマト葉の感染状態を、PVAハイドロゲルによる蛍光物質の非破壊抽出・検出で早期推定する手法の開発が中心であり、植物病害状態のフェノタイピングに該当する。

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