Development of bright fluorescent auxin

Abstract

Polar transport of the phytohormone auxin plays a crucial role in plant growth and response to environmental stimuli. Small-molecule tools that visualize auxin distribution in intact plants enable us to understand how plants dynamically regulate auxin transport to modulate growth. In this study, we developed a new fluorescent auxin probe, BODIPY-IAA2, which effectively visualizes auxin distribution in various plant tissues. We designed this probe to be transported by auxin transporters while lacking the ability to elicit auxin signaling. Using BODIPY as the fluorophore provides bright and stable fluorescence signals, making it suitable for live-imaging under standard fluorescent microscopy. We tested the probe with auxin reporter lines in Arabidopsis and performed yeast two-hybrid assays. The results showed that BODIPY-IAA2 did not activate auxin signaling through the auxin receptor TIR1. However, BODIPY-IAA2 did mildly compete with both exogenous and endogenous auxins for transport, indicating that the probe is transported by auxin transporters in vivo. The probe not only enables visualization of its tissue distribution but also allows sub-cellular staining, including the endoplasmic reticulum and tip regions in elongating cells in moss. We also observed unusual staining patterns in the main root of non-model parasitic plants where genetic transformation is not feasible. Our new fluorescent auxin probe demonstrates significant potential for detailed studies on auxin transport and distribution across diverse plant species.

Plant phenotyping relevance

植物体内のオーキシン分布を可視化する蛍光プローブを開発し、植物組織・細胞でのライブイメージング性能を検証しており、表現型取得手法が研究の中心である。

Code and data availability

The supplied blocks describe fluorescent auxin probe development and microscopy/phenotype assays (root elongation, gravitropism, GUS staining) but contain no data availability statement, deposited phenotype datasets, image collections, analysis code, or trained models. No public paper-specific asset is identified.

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