Quick Histochemical Staining Methods to Detect Cell Death in Xylem Elements of Plant Tissues.

Abstract

Histochemical assays of xylem cell death cannot take advantage of the conventional methods for detection of cell death, such as staining with propidium iodide or trypan/Evans blue or the TUNEL staining. This chapter presents two alternative histochemical methods that can be used to detect xylem cell death quickly and reliably using light microscopy. The first method is a viability stain that can be used to detect cell death of different types of xylem elements in basically any plant species. The second method reveals cell death in xylem vessel elements based on their functionality in transport of water and small water-soluble stains.

Plant phenotyping relevance

植物組織の木部細胞死を光学顕微鏡で検出する2つの組織化学的手法が中心であり、植物の状態を取得する方法論として収録対象。

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