Detection of Reactive Oxygen and Nitrogen Species (ROS/RNS) During Hypersensitive Cell Death.

Abstract

Reactive oxygen and nitrogen species (ROS/RNS) are signaling molecules involved in a plethora of physiological processes in plants. Especially, ROS and nitric oxide (NO) are key players that are required for programmed cell death (PCD). The PCD associated with the hypersensitive response (HR) has been well characterized and the role of H 2 O 2 and NO as key signaling molecules inducing HR has been established. Localization of ROS and NO production in plant tissues in response to pathogens can be imaged by confocal laser microscopy by using specific fluorescent probes. Deciphering the time and spatial regulation of ROS and NO is very important to establish the cellular response of plants to adverse conditions. This chapter is mainly focused on the imaging of ROS and RNS accumulation in vivo in plant tissues undergoing PCD.

Plant phenotyping relevance

植物組織におけるROS/RNS蓄積を蛍光プローブと共焦点顕微鏡で可視化する手法を中心に扱う方法論章であり、植物の生理状態の取得・評価が主題である。

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