Live Fluorescence Visualization of Cellulose and Pectin in Plant Cell Walls.

Abstract

The plant cell wall comprises various types of macromolecules whose abundance and spatial distribution change dynamically and are crucial for plant architecture. High-resolution live cell imaging of plant cell wall components is, therefore, a powerful tool for plant cell biology and plant developmental biology. To acquire suitable data, the experimental setup for staining and imaging of non-fixed samples must be straightforward and avoid creating stress-induced artifacts. We present a detailed sample preparation and live image acquisition protocol for fluorescence visualization of cell wall components using commercially available probes and stains.

Plant phenotyping relevance

植物細胞壁成分の空間分布を取得するためのライブ蛍光染色・画像取得プロトコルが研究の中心であり、単なる生物学的測定ではない。

Code and data availability

This is a methods protocol chapter describing live fluorescence staining and confocal imaging of plant cell walls. No public phenotype/trait datasets, image deposits, author analysis code, trained models, or supplements with such assets are mentioned anywhere in the supplied blocks. The only referenced resources are a染

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