Tracking Nano- and Microplastics in Plants: Uptake Pathways, Tissue Distribution, and Analytical Strategies from Microscopy to Spectroscopy.

Abstract

Nano- and microplastics (NMPs) are now widely detected across agroecosystems and can act as physiological stressors in plants. Exposure occurs through contaminated soil, irrigation water, or airborne deposition, bringing particles into direct contact with roots and above-ground tissues. Reported entry routes include apoplastic transport, cracks formed at lateral root emergence, leaf stomata, and endocytosis once particles have crossed the cell wall. Once internalized, particles may translocate through the xylem and, in some cases, the phloem, accumulating in roots, stems, and leaves depending on particle size, surface charge, and plant structural characteristics. NMPs have been associated with oxidative stress, disrupted photosynthesis, and altered metabolic pathways. Detecting NMPs within heterogeneous, hydrated plant tissues remains challenging, as particles often show low contrast against biological structures and can be mistaken for cellular components. This review examines how microscopy techniques reveal NMPs size, surface attachment, tissue distribution, and cellular-level interactions, while noting that these approaches primarily provide morphological or localization information rather than confirming polymer identity. Complementary spectroscopic and mass-based analytical methods are discussed for their role in chemical confirmation and quantification. This review supports informed selection among imaging, spectroscopic, and quantitative techniques for studying plant-plastic interactions, while highlighting current analytical challenges facing the field.

Plant phenotyping relevance

植物組織内の粒子サイズ・付着・分布・細胞相互作用を測定する顕微鏡、分光、質量分析手法を中心にレビューしており、植物状態の観測手法が主題である。

Code and data availability

This is a narrative review of nano-/microplastics in plants with no original phenotyping measurements, datasets, images, or analysis code. The only URL mentioned (illustrae.co) is a commercial scientific illustration platform used to draw figures, not a paper-specific data, image, or code asset. No data availability or

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