Root Gravitropism: Quantification, Challenges, and Solutions.

Abstract

Better understanding of root traits such as root angle and root gravitropism will be crucial for development of crops with improved resource use efficiency. This chapter describes a high-throughput, automated image analysis method to trace Arabidopsis (Arabidopsis thaliana) seedling roots grown on agar plates. The method combines a "particle-filtering algorithm with a graph-based method" to trace the center line of a root and can be adopted for the analysis of several root parameters such as length, curvature, and stimulus from original root traces.

Plant phenotyping relevance

根の画像から中心線を自動追跡し、長さ・曲率・根角度などの形態形質を高スループットに定量する画像解析法が中心である。

Code and data availability

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