A simple and cost-effective method for studying anoxia tolerance in plants.

Abstract

Premise We developed a novel, cost-effective protocol that facilitates testing anoxia tolerance in plants without access to specialized equipment. Methods and results Arabidopsis thaliana and barley ( Hordeum vulgare ) seedlings were treated in airtight 2-L Kilner jars. An anoxic atmosphere was generated using Oxoid AnaeroGen 2.5-L sachets placed on in-house, custom-built wire stands. The performed experiments confirmed a higher sensitivity to low oxygen stress previously observed in anac017 A. thaliana mutants and the positive effect of exogenous sucrose on anoxia tolerance reported by previous studies in A. thaliana . Barley seedlings displayed typical responses to anoxia treatment, including shoot growth cessation and the induction of marker genes for anaerobic metabolism and ethylene biosynthesis in root tissue. Conclusions The results validate the novel method as an inexpensive, simple alternative for testing anoxia tolerance in plants, where access to an anaerobic workstation is not possible. The novel protocol requires minimum investment and is easily adaptable.

Plant phenotyping relevance

植物の無酸素耐性を評価するための新規・低コストな実験プロトコルを開発し、植物の成長停止などの表現型応答で妥当性を検証しており、方法が研究の中心です。

Code and data availability

The article describes a low-cost anoxia tolerance protocol with survival, shoot growth, and qPCR measurements, but contains no public phenotype dataset, image/sensor data, analysis code, or trained model deposit. Appendices are only materials lists and protocol text. No author-provided public URL for paper-specific raw

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