Cell Biological Analyses of Anther Morphogenesis and Pollen Viability in Arabidopsis and Rice.

Abstract

Major advances have been made in our understanding of anther developmental processes in flowering plants through a combination of genetic studies, cell biological technologies, biochemical analyses, microarray and high-throughput sequencing-based approaches. In this chapter, we summarize widely used protocols for pollen viability staining, investigation of anther morphogenesis by scanning electron microscopy (SEM), light microscopy of semi-thin sections, ultrathin section-based transmission electron microscopy (TEM), TUNEL (terminal deoxynucleotidyl transferase-mediated 2'-deoxyuridine 5'-triphosphate (dUTP) nick end labeling) assay for tapetum programmed cell death, and laser microdissection procedures to obtain specific cells or cell layers for transcriptome analysis.

Plant phenotyping relevance

花粉生存性や葯の形態を観察・評価する複数の植物表現型取得プロトコルを体系的に扱う方法論的章であり、測定手法が中心である。

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