Going cresyl for plant cell imaging

Abstract

The advent of fluorescent probes and the characterization of their photochemical properties in the past years allowed significant advances in the studies of spatiotemporal cellular processes within complex and crowded systems. Dyes are indeed extremely useful tools for the visualization of cellular and subcellular structures present in living cells, as well as to study their dynamic and molecular composition or physiological changes. There are some areas of plant cell biology that have been more challenging to explore due to the physiology and organization of certain endomembrane compartments. In this study we characterize the labeling properties of cresyl violet as quick and inexpensive imaging agent for tracking endosomes, vacuole compartments which are usually very differentiated and categorized as more acidic as well as acidic plant compartments. Its photobleaching, labelling and cytotoxic properties are compared with other well-known and currently most used synthetic and molecular probes.

Plant phenotyping relevance

植物細胞内区画の可視化・追跡用蛍光プローブを開発・特性評価し、既存プローブと比較しているため、植物状態の画像取得法が中心です。

Code and data availability

The article describes cresyl violet characterization in plant cells via confocal microscopy, but no public phenotype dataset, image collection, analysis code, or trained model is deposited. The only availability statements are 'Data will be made available on request' and biological materials via ABRC stock center (a re

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