Biomechanical Characterization of Onion Epidermal Cell Walls.

Abstract

Here we describe two experimental protocols to measure the biomechanical properties of primary (growing) plant cell walls, with a focus on analyzing cell wall epidermal strips of onion scales. The first protocol measures cell wall creep (time-dependent irreversible extension) under constant force. Such creep is often mediated by the wall-loosening action of expansin or selective endoglucanases. The second protocol is based on two consecutive stretches of the wall and measures the wall's elastic and plastic compliances, which depend on cell wall structure. These two assays provide complementary information that may be linked to cell wall structure and expansive growth of cells.

Plant phenotyping relevance

植物細胞壁の力学的性質を測定する2つの実験プロトコル自体が中心であり、細胞壁構造や伸長成長に関わる植物状態を定量化するため、方法論文として収録する。

Code and data availability

This is a methods/protocol paper describing onion epidermal cell wall biomechanics assays. It mentions custom software written in Microsoft Visual Basic for data acquisition and analysis, but provides no public deposit, repository, or authors' URL for that code; the only URL given (http://www.microsoft.com) is a link,

No evidence-backed public reproduction asset is currently recorded.