C13MP, a method using C13 isotope labeling to measure plant photosynthetic rate precisely.

Abstract

Photosynthesis is the cornerstone of life on earth. Precisely evaluating the photosynthetic rate is critical to uncovering its underlying mechanisms. Numerous methods and tools have been developed to assess plant photosynthetic rate. However, these methods typically measure the carbon assimilation rate indirectly during photosynthesis, highlighting the need for a new method to determine plant photosynthetic rate precisely. This study developed an easy and reliable method using 13 C isotope-labeled CO 2 and isotope element spectrometer technology to measure plant photosynthetic rate (C13MP). C13MP can precisely quantify the abundance of assimilated carbon by photosynthesis in plants. C13MP can also evaluate the carbon flow from leaves to the other organs by tracking the 13 C isotope. The photosynthetic rates determined with C13MP were significantly correlated with maize biomass-related and yield-related phenotypes, suggesting the reliability of this method. In conclusion, C13MP can easily, precisely, and reliably evaluate carbon assimilation in various parts of the plant, providing a new method for measuring plant photosynthetic rate. Supplementary information The online version contains supplementary material available at 10.1007/s11032-025-01601-0.

Plant phenotyping relevance

植物の光合成速度と炭素移行を定量する新規測定法を開発し、作物表現型との相関で信頼性を検証しており、フェノタイピング手法が中心である。

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