Estimation of Silica Cell Silicification Level in Grass Leaves Using in situ Charring Method.

Abstract

Silica cells are specialized leaf epidermal cells in grasses with almost the whole cell volume filled with solid silica. In sorghum, silica deposition in silica cells takes place in young, elongating leaves around the mid-length of the leaf. We developed a protocol for estimating the level of silica cell silicification in Sorghum bicolor leaves using in situ charring method ( Kumar et al. , 2017a ). Here, we provide greater details on our protocol and method of image analysis. Although we based our protocol on sorghum, this protocol can be extended for estimating silica cell silicification level in any grass species.

Plant phenotyping relevance

イネ科葉の珪化レベルという植物形質を推定するプロトコルと画像解析法を詳細化しており、形質取得法が中心である。

Code and data availability

This is a Bio-protocol methods article describing an in situ charring/spodogram protocol for estimating silica cell silicification in sorghum leaves. The supplied blocks contain no public phenotype dataset, image collection, analysis code, or model deposit; no availability statements or URLs for paper-specific assets (

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