Novel dye for detection of callus embryo by confocal laser scanning fluorescence microscopy.

Abstract

In the present study a new luminescent dye 3-N-(2-pyrrolidinylacetamido)benzanthrone (AZR) was synthesized. Spectroscopic measurements of the novel benzanthrone 3-aminoderivative were performed in seven organic solvents showing strong fluorescence. The capability of the prepared dye for visualization has been tested on flax, red clover and alfalfa to determinate the embryo in plant callus tissue cultures. Callus cells were stained with AZR and further analysed utilizing confocal laser scanning fluorescence microscopy. Performed experiments show high visualization effectiveness of newly synthesized fluorescent dye AZR that is efficient in fast and relatively inexpensive diagnostics of callus embryos that are problematic due to in vitro culture specificity.

Plant phenotyping relevance

新規蛍光色素と共焦点顕微鏡を用いて植物カルス中の胚を可視化・診断する手法が研究の中心であり、植物の発生状態を取得する画像ベースのフェノタイピング手法に該当する。

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