A Plate Growth Assay to Quantify Embryonic Root Development of Zea mays .

Abstract

Murashige-Skoog medium solutions have been used in a variety of plant plate growth assays, yet most research uses Arabidopsis thaliana as the study organism. For larger seeds such as maize ( Zea mays ), most protocols employ a paper towel roll method for experiments, which often involves wrapping maize seedlings in wet, sterile germination paper. What the paper towel roll method lacks, however, is the ability to image the roots over time without risk of contamination. Here, we describe a sterile plate growth assay that contains Murashige-Skoog medium to grow seedlings starting two days after germination. This protocol uses a section of a paper towel roll method to achieve uniform germination of maize seedlings, which are sterilely transferred onto large acrylic plates for the duration of the experiment. The media can undergo modification to include an assortment of plant hormones, exogenous sugars, and other chemicals. The acrylic plates allow researchers to freely image the plate without disturbing the seedlings and control the environment in which the seedlings are grown, such as modifications in temperature and light. Additionally, the protocol is widely adaptable for use with other cereal crops. Key features • Builds upon plate growth methods routinely used for Arabidopsis seedlings but that are inadequate for maize. • Real-time photographic analysis of seedlings up to two weeks following germination. • Allows for testing of various growth conditions involving an assortment of additives and/or modification of environmental conditions. • Samples are able to be collected for genotype screening.

Plant phenotyping relevance

トウモロコシ幼苗の根発達を経時的に画像化・定量するプレートアッセイ自体を開発・提示しており、植物表現型取得が中心である。

Code and data availability

This is a methods/protocol article describing a plate growth assay for maize root phenotyping. The paper uses generic third-party software (ImageJ, SmartRoot, a PDF-to-JPG converter) for analysis, but no public phenotype/trait datasets, author-deposited images, analysis code, or trained models are mentioned. No data or

No evidence-backed public reproduction asset is currently recorded.