DNA content equivalence in haploid and diploid maize leaves.

Abstract

Main conclusion The qPCR assay developed to differentiate haploid and diploid maize leaf samples was unsuccessful due to DNA content difference. Haploid cells are packed more closely together with less cellular expansion. Increased ploidy content (> 2 N) directly correlates with increased cell size in plants, but few studies have examined cell morphology in plants with reduced ploidy (i.e., haploids). To pioneer a scalable new ploidy test, we compared DNA content and cellular morphology of haploid and diploid maize leaves. The amount of genomic DNA recovered from standardized leaf-punch samples was equivalent between these two ploidy types, while both epidermal and mesophyll cell types were smaller in haploid plants. Pavement cells had a substantially smaller size than mesophyll cells, and this effect was more pronounced in the abaxial epidermis. Interveinal distance and guard cell size were significantly reduced in haploids, but the cell percentage comprising stomata did not change. These results confirm the direct correlation between ploidy content and cell size in plants, and suggest that reduced cell expansion predominantly explains DNA content equivalence between haploid and diploid samples, confounding efforts to develop a haploid detection method using DNA content.

Plant phenotyping relevance

ハプロイド／二倍体という植物状態の判別法を開発・評価し、DNA量と葉の細胞形態を測定して手法の失敗要因も検証しているため、方法開発・検証が中心です。

Code and data availability

The article describes qPCR DNA quantification and confocal microscopy of maize leaf disks, but no public phenotype dataset, image collection, analysis code, or supplement with such assets is mentioned. The only URLs present are a cited JRC qPCR method report (prior work), a commercial equipment vendor site (MEKU), the

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