Aptamer-Based Microfluidic Assay for In-Field Detection of Salicylic Acid in Botrytis cinerea -Infected Strawberries.

Abstract

Rapid detection of plant infections is crucial for minimising crop loss and optimising management strategies, particularly in the context of climate change. While traditional diagnostic methods provide precise measurements of phytohormones such as salicylic acid (SA), a key regulator of plant defence responses, their reliance on bulky equipment and lengthy analysis times limits field applicability. This study presents a microfluidic-based aptamer assay for SA detection, enabling rapid and sensitive fluorescence-based readout from plant samples. A tailored sample pre-treatment protocol was developed and validated with real strawberry samples using HPLC measurements. The assay demonstrated a detection limit ranging from 10 -9 to 10 -6 mg/mL, within the relevant range for early infection diagnosis. The integration of the microfluidic platform with the optimised pre-treatment protocol offers a portable, cost-effective solution for on-site phytohormone analysis, providing a valuable tool for early infection detection and improved crop management.

Plant phenotyping relevance

植物試料中のサリチル酸を対象とするマイクロ流体・アプタマー測定法を開発し、実試料とHPLCで検証しており、感染に関連する植物の生理状態の取得が中心である。

Code and data availability

植物フェノタイピング解析を再現する公開資産であることを、入力本文と直接リンクから確認できなかったため保留しました。

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